Rate difference for restriction enzyme EcoRI endonuclease activity for cognate and next best site (ideal conditions)
| Value | 100000 unitless |
|---|---|
| Organism | Generic |
| Reference | link |
| Primary Source | Lesser, D.R., Kurpiewski, M.R. and Jen-Jacobson, L. (1990) The energetic basis of specificity in the EcoR I endonucleaseDNA interaction. Science 250, 776–86.PubMed ID2237428 |
| Comments | under ideal conditions. However, under nonoptimal conditions, the differences in cleavage rates between cognate and next-best sites change dramatically for many enzymes. This loss of fidelity or increase in cleavage at sites similar to the cognate site is commonly referred to as star activity. |
| Entered by | Ron Milo - Admin |
| ID | 101485 |