| Value |
0.34
mM
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Koç A, Wheeler LJ, Mathews CK, Merrill GF. Hydroxyurea arrests DNA replication by a mechanism that preserves basal dNTP pools. J Biol Chem. 2004 Jan 2 279(1):223-30 DOI: 10.1074/jbc.M303952200 p.229 right column bottom paragraphPubMed ID14573610
|
| Primary Source |
[40] Exinger F, Lacroute F. 6-Azauracil inhibition of GTP biosynthesis in Saccharomyces cerevisiae. Curr Genet. 1992 Jul22(1):9-11 [41] Larsson C et al., Glycolytic flux is conditionally correlated with ATP concentration in Saccharomyces cerevisiae: a chemostat study under carbon- or nitrogen-limiting conditions. J Bacteriol. 1997 Dec179(23):7243-50PubMed ID1611672, 9393686
|
| Method |
Calculated from published data |
| Comments |
P.229 right column bottom paragraph: "The mechanism for replication arrest when RNR [ribonucleotide reductase] is inhibited remains unknown. If RNR inhibition resulted in significant expansion of the rNTP pools, it is possible that an increased rNTP/dNTP ratio might result in misincorporation of ribonucleotides into DNA and thereby trigger a replication arrest. However, the rNTP pools in yeast, calculated from published data to be 0.34 mM rUTP, 2 mM rATP, 0.26 mM rCTP, and 0.34 mM rGTP (primary sources), are 5–90-fold greater than the corresponding dNTP pools (Fig. 1B) (refs 15, 18) and are therefore unlikely to be affected by the presence or absence of a relatively small flux of rNDPs through RNR." |
| Entered by |
Paul Jorgensen |
| ID |
101420 |