| Value |
1136
pores/nucleus
|
| Organism |
Fruit fly Drosophila melanogaster |
| Reference |
Maul GG, Deaven L. Quantitative determination of nuclear pore complexes in cycling cells with differing DNA content. J Cell Biol. 1977 Jun73(3):748-60. p. 752 table IPubMed ID406262
|
| Method |
P.749 right column top paragraph: "Drosophila cells grew at 25°C in Schneider's medium with 10% heat-inactivated serum." P.749 right column 2nd paragraph: "Determinations of nuclear diameter by phase-contrast microscopy, of height by electron microscopy, and of pore frequency by freeze-etching were done on cells during the exponential growth phase (48 h after plating)." P.753 right column 3rd paragraph: "[Investigators] decided to extend the number of different cell lines and select them according to different DNA content. Table I provides the summary of the data collected during this extended investigation, and reveals an obvious increase in nuclear surface with increasing DNA content: this increase is not proportional, however." |
| Comments |
P.751 right column 2nd paragraph: "The numbers in Table I represent the corrected values. Briefly, the indentation at the cytocenter results in a nuclear shape similar to the shape of an indented ball. Since most cells will appear round when the indentation is parallel to the optical axis, the volume will be extremely overestimated if the formula for a sphere is used." (S2) imaginal disc |
| Entered by |
Ben Marks |
| ID |
101394 |