Number of nuclear pores in embryo tissue nuclei
|Frog Rana pipiens
|Maul GG, Deaven L. Quantitative determination of nuclear pore complexes in cycling cells with differing DNA content. J Cell Biol. 1977 Jun73(3):748-60. p. 2124 table 1PubMed ID406262
|Cells were established and maintained in Leibowitz medium, with 20% fetal calf serum diluted to 60% with H2O at 24°C. Determinations of nuclear diameter by phase-contrast microscopy, of height by electron microscopy, and of pore frequency by freeze-etching were done on cells during the exponential growth phase (48 h after plating). For determination of nuclear size the short and long axis of 50 live cells were measured and averaged separately.