| Value |
71.6
nucleotides/sec
|
| Organism |
Mammalian tissue culture cell |
| Reference |
Darzacq X, Shav-Tal Y, de Turris V, Brody Y, Shenoy SM, Phair RD, Singer RH. In vivo dynamics of RNA polymerase II transcription. Nat Struct Mol Biol. 2007 Sep14(9):796-806 abstract & p.803 right column top paragraph & fig.9aPubMed ID17676063
|
| Method |
P.796 right column 2nd paragraph: "Here [investigators] report a method for the in vivo labeling of mRNA transcripts containing a series of repeated stem-loops (from phage MS2), which are specifically bound by an MS2 coat protein fused to green fluorescent protein (GFP)[ref 16]. The assay consists of a human cell line harboring a gene array into which these stem-loops have been integrated [ref 17]." |
| Comments |
Abstract: "Polymerases elongated at 4.3 kilobases/min, much faster than previously documented, and entered a paused state for unexpectedly long times." P.803 right column top paragraph: "A nonpausing polymerase could transcribe the entire 3.3-kb gene in 46 s (at 4.3 kb/min)." (value is 4.3kb/min) See also average polymerase velocity (BNID 100661) and pause residence time (BNID 104586). YFP-Pol II is amanitin resistant allele. See BNID 101904, 100060 |
| Entered by |
Ian Swinburne |
| ID |
100662 |