RNA polymerase II transcription elongation rate in HeLa cells

Range 50 -100 nucleotides/sec
Organism Human Homo sapiens
Reference Sehgal PB, Derman E, Molloy GR, Tamm I, Darnell JE. 5,6-Dichloro-1-Beta-D-ribofuranosylbenzimidazole inhibits initiation of nuclear heterogeneous RNA chains in HeLa cells. Science. 1976 Oct 22 194(4263):431-3. abstractPubMed ID982026
Method p.432 middle column 3rd paragraph:"Cells were treated with DRB [5,6-Dichloro-1-Beta-D-ribofuranosylbenzimidazole] For varying times, and exposed to [3H]uridine For 45 seconds the sedimentation profile of hnRNA was determined as described (refs 4,5). The effect of DRB on uridine transport was eliminated from consideration by comparing the radioactivity (counts Per minute) in the sucrose gradient samples as fractions of total acid-precipitable radioactivity recovered, not the incorporated radioactivity per se."
Comments Abstract:"By progressively increasing the time of exposure to DRB, and measuring the rate of increase in the average size of the labeled, nascent RNA, it was estimated that the chains were growing at rates between 50 and 100 nucleotides per second."
Entered by Uri M
ID 111721