Lignin as percent of cell wall

Range 13-28 %
Organism Plants
Reference Sykes R, Yung M, Novaes E, Kirst M, Peter G, Davis M. High-throughput screening of plant cell-wall composition using pyrolysis molecular beam mass spectroscopy. Methods Mol Biol. 2009 581: 169-83.PubMed ID19768623
Method Researchers describe a high-throughput method for estimating cell-wall chemistry traits using analytical pyrolysis. The instrument used to perform the high-throughput cell-wall chemistry analysis consists of a commercially available pyrolysis unit and autosampler coupled to a custom-built molecular beam mass spectrometer. The system is capable of analyzing approximately 42 biomass samples per hour. Lignin content and syringyl to guaiacol (S/G) ratios can be estimated directly from the spectra and differences in cell wall chemistry in large groups of samples can easily be identified using multivariate statistical data analysis methods. A custom-built molecular-beam mass spectrometer using an Extrel Model TQMS C50 mass spectrometer was used for pyrolysis vapor analysis (15, 22). The poplar trees used are the progeny of a cross between a Populus trichocarpa x Populus deltoides hybrid (clone 52–225) and a P. deltoides pure genotype (clone D124).
Comments High-throughput analytical pyrolysis was able to determine that the lignin content varied between 13 and 28% and the S/G ratio ranged from 0.5 to 1.5.
Entered by Ron Milo - Admin
ID 100588