|Sykes R, Yung M, Novaes E, Kirst M, Peter G, Davis M. High-throughput screening of plant cell-wall composition using pyrolysis molecular beam mass spectroscopy. Methods Mol Biol. 2009 581: 169-83.PubMed ID19768623
|Researchers describe a high-throughput method for estimating cell-wall chemistry traits using analytical pyrolysis. The instrument used to perform the high-throughput cell-wall chemistry analysis consists of a commercially available pyrolysis unit and autosampler coupled to a custom-built molecular beam mass spectrometer. The system is capable of analyzing approximately 42 biomass samples per hour. Lignin content and
syringyl to guaiacol (S/G) ratios can be estimated directly from the spectra and differences in cell wall
chemistry in large groups of samples can easily be identified using multivariate statistical data analysis
methods. A custom-built molecular-beam mass spectrometer using an
Extrel Model TQMS C50 mass spectrometer was used for pyrolysis vapor analysis (15, 22). The poplar trees used are the progeny of a cross between a Populus trichocarpa x Populus deltoides hybrid (clone 52–225) and a P. deltoides pure genotype (clone D124).
|High-throughput analytical pyrolysis was able to determine that the lignin content varied between 13 and 28% and the S/G ratio ranged from 0.5 to 1.5.
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