Diameter of monocyte

Value 8.79 µm Range: 7.72-9.99 Table - link µm
Organism Human Homo sapiens
Reference Downey GP, Doherty DE, Schwab B 3rd, Elson EL, Henson PM, Worthen GS. Retention of leukocytes in capillaries: role of cell size and deformability. J Appl Physiol. 1990 Nov69(5):1767-78 p.1771 table 1PubMed ID2272970
Method P.1768 right column bottom paragraph: "The measurement of the size of isolated and purified leukocytes was done by two independent methods. The first method of leukocyte size estimation (5, 34) utilized axial light extinction in the pulse width mode on a cytofluorograph (model 50H with appended 2150 Computer, Ortho Diagnostics, Westwood, MA) calibrated with standard 5-, 10-, and 15-µm latex beads (Coulter). This is a measurement of the time required for the cell to cross the laser beam and provides a measurement of cell diameter (ref 34), provided the cells are predominantly spherical, which is valid for unactivated leukocytes." P.1769 left column 2nd paragraph: "The second method employed a Coulter counter calibrated with latex beads of known size."
Comments P.1771 left column bottom paragraph: "Size measurements of leukocytes: To correlate leukocyte retention with cell size, leukocyte diameter was measured in two ways (Table 1, Fig. 2): by use of axial light extinction in the pulse width mode on a flow cytometer (refs 5,34) and with a Coulter counter (ref 40). As measured by flow cytometer, neutrophils were the largest cells with a mean diameter of 8.3µm, lymphocytes the smallest with a mean diameter of 6.05µm, and monocytes intermediate with a mean diameter of 8.13µm. These results were confirmed by the Coulter counter and did not appear to change significantly when the cells were fixed and dehydrated (Table 1). Because the measured diameters of the leukocytes were significantly larger than those reported by other investigators who used morphometry on fixed leukocytes (ref 40), [investigators] questioned whether [their] isolation procedure per se (plasma-Percoll) resulted in an (artificial) increase in cell size." Value represents mean of coulter counter measurements of 11,000-70,000 cells from three donors. Neutrophil diameter measured from same samples by flow cytometer is 8.13µm, range 7.17-9.3µm. See table link for diameter of neutrophils and lymphocytes. see BNID 100510
Entered by Paul Jorgensen
ID 100508