Absolute abundance of p53 in cell

Range ~160,000 (21,000 to 195,700) Copies/cell
Organism Human Homo sapiens
Reference Ma L, Wagner J, Rice JJ, Hu W, Levine AJ, Stolovitzky GA. A plausible model for the digital response of p53 to DNA damage. Proc Natl Acad Sci U S A. 2005 Oct 4 102(40):14266-71. Supplementary text - link p.9 2nd paragraph PubMed ID16186499
Method Supplementary text p.10 2nd paragraph: "The basal concentration of p53 was measured using human p53 Elisa kit (Biosource, Camarillo, CA) according to the manufacturer’s instruction." Griffin et al., Human Anti-antibody Response, p.508 2nd paragraph:"ELISA [Enzyme-linked immunosorbant assay]...is a sensitive immunoassay that uses an enzyme linked to an antibody or antigen as a marker for the detection of a specific protein, especially an antigen or antibody."
Comments Supplementary text p.9 2nd paragraph: "The p53 concentration of each cell line was measured 3 times. As shown in Fig. 12, the p53 concentrations in various cell lines range from 2.1-19.57×10^4 molecules per cell. Specifically, the basal p53 concentration in MCF7 cell is 15.84×10^4 molecules per cell." Value is for MCF7 breast cancer cell line. Range is for A8750, MCF7, H460, Tera2, LB19, LB43,,WI38 cell lines. Concentration ranges from 0.06 to 0.5µM depending on cell line. See BNID 106910
Entered by Alex Loewer
ID 100420