p53 concentration in wildtype liver cell L-02

Value 2.87 nM Range: ±0.043 Table - link nM
Organism Human Homo sapiens
Reference Wang Y, Zhu X, Wu M, Xia N, Wang J, Zhou F. Simultaneous and label-free determination of wild-type and mutant p53 at a single surface plasmon resonance chip preimmobilized with consensus DNA and monoclonal antibody. Anal Chem. 2009 Oct 15 81(20):8441-6. p.8445 table 1PubMed ID19772286
Method Abstract: "Simultaneous determination of wild-type and total p53 proteins (wild-type and mutant combined) present in cancer cell lysates has been performed with a dual-channel surface plasmon resonance (SPR) instrument. To achieve specificity, each channel of the SPR chip was modified with a consensus double-stranded (ds-) DNA and a monoclonal antibody."
Comments P.8446 left column bottom paragraph: "The mutant p53 concentration can be deduced from the difference between wild-type and total p53 (Table 1)."
Entered by Uri M
ID 106632