| Value |
48
Unitless
|
| Organism |
Chimpanzee Pan troglodytes |
| Reference |
Young WJ, Merz T, Ferguson-Smith MA, Johnston AW. Chromosome number of the chimpanzee, Pan troglodytes.
Science. 1960 Jun 3 131:1672-3.PubMed ID13846659
|
| Method |
Researchers have been able to ascertain the
chromosome numbers of nine individuals of Pan troglodytes (seven males
and two females), which were sacrificed
because of infection with tuberculosis
(3). Anesthesia was induced with ether
and maintained for 3 to 5 hours with
Nembutal. This period afforded sufficient time for the action of the mitotic
poisons used: either colchicine, 0.25
mg/ kg injected intraperitoneally, or
Colcemid (4), 6 mg per animal injected
intravenously.
Bone marrow was obtained from the proximal third of the humerus (in one
case from the radius) it was suspended
in 1.12 percent sodium citrate at 37°C
for 20 to 30 minutes, centrifuged, and
either fixed in cold alcohol-acetic acid
and prepared by the Feulgen squash
method (5) or fixed in 50 percent acetic
acid and stained with lactic-acetic
orcein and then squashed (6). Counts of
suitable metaphase plates were made
directly from the preparations. |
| Comments |
The chromosome numbers of nine chimpanzees have been determined by the bone marrow technique. The diploid number in this species is 48, with a probable XX-XY sex chromosome constitution. Notice that this is the 2n value, i.e. counting the sister chromosomes as two separate chromosomes. See BNID 100371 |
| Entered by |
Ron Milo - Admin |
| ID |
100323 |