Volume of 70S (intact) ribosome according to chemical molecular mass

Value 2680 nm^3
Organism Bacteria Escherichia coli
Reference Gabashvili IS, Agrawal RK, Spahn CM, Grassucci RA, Svergun DI, Frank J, Penczek P. Solution structure of the E. coli 70S ribosome at 11.5 A resolution. Cell. 2000 Mar 3 100(5):537-49 p.538 left column 2nd paragraphPubMed ID10721991
Primary Source Wittmann HG, Müssig J, Piefke J, Gewitz HS, Rheinberger HJ, Yonath A. Crystallization of Escherichia coli ribosomes. FEBS Lett. 1982 Sep 6 146(1):217-20PubMed ID6754439
Method Over 73,000 projections of the E. coli ribosome bound with formyl-methionyl initiator tRNAfMet were used to obtain an 11.5 Å cryo-electron microscopy map of the complex. For surface representations, threshold values were selected using the molecular volume as a guide. The protein level was initially chosen to encompass the total volume of 2.6 × 106 Å3, based on the chemical molecular mass (Primary source). Researchers used a slightly enlarged volume of 2.68 × 10^6 Å3 to retain continuity of some structural features.
Comments See BNID 102475 (Solvent-excluded Volume of 50S ribosome)
Entered by Ron Milo, Paul Jorgensen, Mike Springer
ID 100122