Fraction of expressed proteins that accumulate rhythmically during the day in liver & SCN [suprachiasmatic nuclei]

Range 10 - 20 %
Organism Mouse Mus musculus
Reference Mauvoisin D et al., Circadian clock-dependent and -independent rhythmic proteomes implement distinct diurnal functions in mouse liver. Proc Natl Acad Sci U S A. 2014 Jan 7 111(1):167-72. doi: 10.1073/pnas.1314066111. p.167 right column top paragraphPubMed ID24344304
Primary Source [9] Reddy AB, et al. (2006) Circadian orchestration of the hepatic proteome. Curr Biol 16(11):1107–1115. [10] Deery MJ, et al. (2009) Proteomic analysis reveals the role of synaptic vesicle cycling in sustaining the suprachiasmatic circadian clock. Curr Biol 19(23):2031–2036.PubMed ID16753565, 19913422
Method 2D gel electrophoresis
Comments p.167 left column bottom paragraph:"However, comparatively little is known on the temporal accumulation of proteins, despite increasing evidence suggesting that posttranscriptional mechanisms also contribute to circadian rhythms at the protein level (ref 5), including mRNA translation (refs 6–8). Of interest, two medium-scale studies relying on 2D gel electrophoresis to quantify the liver and SCN proteomes in mouse concluded that around 10–20% of expressed proteins accumulate rhythmically during the day (primary sources). Surprisingly, in both cases, many of the rhythmic proteins were encoded by nonrhythmic mRNAs, highlighting that translational or posttranslational circadian regulation underlies the accumulation of the circadian proteome."
Entered by Uri M
ID 111859