Number of protons that are required by the F-ATPase to make each ATP molecule in vertebrates and invertebrates (equivalent to 8 protons translocated each rotation)

Value 2.7 unitless
Organism Eukaryotes
Reference Watt IN, Montgomery MG, Runswick MJ, Leslie AG, Walker JE. Bioenergetic cost of making an adenosine triphosphate molecule in animal mitochondria. Proc Natl Acad Sci U S A. 2010 Sep 16PubMed ID20847295
Method X ray diffraction data were collected from the flash-frozen cryoprotected crystals at the Swiss Light Source in the Paul Scherrer Institut, Villigen, Switzerland. The data were collected at a wavelength of 1.0007 Å on beamline X06SA with a micro-diffractometer and a MAR225 mosaic charge coupled detector (Rayonix).
Comments The most important inference from the presence of the c8-ring in bovine F-ATP synthase, is that eight protons are translocated across the inner mitochondrial membranes per 360° rotation of its rotor. As each 360° rotation produces three ATP molecules from the F1-domain, the bioenergetic cost of the enzyme making an ATP is 2.7 protons. It appears that F-ATPases in all vertebrates and probably all or most invertebrates, will contain c8-rings. Therefore, the bioenergetic cost of the enzyme making an ATP is 2.7 protons in vertebrates and probably in invertebrates also (exceptions dictated by particular energetic demands of a species may exist).
Entered by Burak Okumus
ID 105661