Persistence length of F-actin

Value 17 µm Range: ±2 Table - link µm
Organism Generic
Reference van Mameren J, Vermeulen KC, Gittes F, Schmidt CF. Leveraging single protein polymers to measure flexural rigidity. J Phys Chem B. 2009 Mar 26 113(12):3837-44. p. 3840 right column 3rd paragraph and p.3842 table 1PubMed ID19673071
Method "[Researchers] report a new approach to obtain the flexural rigidity by means of well-controlled active experiments using optical tweezers to bend the filaments locally, as schematically depicted in Figure 1. In this way force-extension curves were measured for taxol-stabilized microtubules and rhodamine-phalloidin labeled actin filaments."
Comments "The persistence length Lp is the exponential decay constant of the thermal tangent-tangent correlation function along the chain, modeled as a homogeneous bendable filament and depends on the flexural rigidity EI of the filament and the temperature T as Lp=EI/kT, where k is the Boltzmann constant." "The average value found for the flexural rigidity of actin is EI=(7.1±0.8)×10^-26nm^2 (standard error of the mean, BNID 105504). This corresponds to a persistence length Lp for actin of 17±2µm using Lp=EI/kBT [Howard, J. Mechanics of motor proteins and the cytoskeleton Sinauer Associates: Sunderland, MA, 2001]." Value in range is standard error of the mean. See BNID 106830
Entered by Uri M
ID 105505