Number of protein coding genes in strain S0385 (livestock-associated MRSA)

Value 2699 ORFs Range: Table - link ORFs
Organism Bacteria Staphylococcus aureus
Reference Schijffelen MJ, Boel CH, van Strijp JA, Fluit AC. Whole genome analysis of a livestock-associated methicillin-resistant Staphylococcus aureus ST398 isolate from a case of human endocarditis. BMC Genomics. 2010 Jun 14 11: 376. p.2 table 1PubMed ID20546576
Method p.8 right column 2nd paragraph:"Annotation [of the genome] was performed using the Annotation Engine, a prokaryotic annotation pipeline developed at the J. Craig Venter Institute (Rockville, MD, USA). A detailed description is available at link cms/research/projects/annotation-service/overview/. In short, the prediction of open reading frames (ORFs) and the identification of structural, tRNA, and tmRNA genes were conducted using Glimmer, tRNAscan-SE, BLAST and the Rfam database. Annotation was performed with BER.BLAST and HMMER against a variety of databases."
Comments "The MRSA [methicillin-resistant Staphylococcus aureus] ST398 genome consists of a circular chromosome of 2,872,582 bp, as well as 3 circular plasmids [sizes of plasmids 1-3 are 5,246bp, 4,381bp and 3,158bp, respectively]. The size of the chromosome is comparable to that of other sequenced S. aureus strains. [Researchers] identified 2,699 ORFs, 6 ribosomal RNA operons, and 24 mobile genetic elements, including 12 insertion sequence (IS) elements. The general features of the genome are summarized in Table 1." Researchers identified 4, 6 and 2 ORFs on plasmids 1, 2, and 3, respectively.
Entered by Uri M
ID 105500