||Fruit fly Drosophila melanogaster
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||Result from Electrophoretic Mobility Shift Assay (EMSA) using isolated GAGA factor ZFD in intermediate ionic strength buffer. EMSA is a shift assay technique which can determine DNA-protein or RNA protein interactions and association/dissociation kinetics. A nucleic acid-protein complex is run in gel next to a control lane that has only nucleic acid. The complex moves slower due to higher mass. Known starting nucleic acid and protein concentrations enable determination of affinity.