| Range |
Table - link
|
| Organism |
Human Homo sapiens |
| Reference |
Kumei Y, Nakajima T, Sato A, Kamata N, Enomoto S. Reduction of G1 phase duration and enhancement of c-myc gene expression in HeLa cells at hypergravity. J Cell Sci. 1989 Jun93 ( Pt 2):221-6. p.224 table 2PubMed ID2693468
|
| Method |
Abstract: "HeLa cells were grown in monolayer in culture flasks that were centrifuged to generate a constant 18, 35 or 70 g at 37 degrees C for up to 4 days. The cell proliferation was enhanced at 18, 35 and 70 g, most notably at 35 g. Cell cycle analyses with [3H]thymidine (TdR)-colcemid treatment showed that the cell generation time in the 35 g culture was reduced by 17% as compared to the control, which was attributed to a 26% reduction of the G1 phase duration." |
| Comments |
P.223 right column 2nd paragraph: "[Researchers] investigated the cell cycle progression of HeLa cells in the 35 g culture by estimating the duration of each cell cycle phase. No significant differences in the duration of the S, G2 or M phase were observed between the 35 g culture and the control (Table 2). However, the duration of the G1 phase was 5.6 ± 0.6 h in the 35 g culture, as compared to 7.6 ± 0.8 h in the control. The cell generation time in the 35 g culture was 13.4 ± 1.3 h, as compared to 16.2 ± 1.7 h in the control. Thus the cell cycle progression of HeLa cells was accelerated at 35 g due to reduction of the G1 phase duration. This reduction of the G1 phase duration predominated the enhancement of HeLa cell proliferation at 35 g." |
| Entered by |
Uri M |
| ID |
103805 |