Stability time of the protein biotin carboxyl carrier protein (BCCP)

Value >= min Range: >60 min
Organism Bacteria Escherichia coli
Reference Li SJ, Cronan JE Jr., Growth rate regulation of Escherichia coli acetyl coenzyme A carboxylase, which catalyzes the first committed step of lipid biosynthesis. J Bacteriol. 1993 Jan175(2):332-40 p.334 right column 2nd paragraphPubMed ID7678242
Method P.334 right column 2nd paragraph: "The stability of BCCP proteins and accBC mRNA was measured by addition of chloramphenicol to cells pulse-labeled with [3H]biotin or by Northern blotting of mRNA isolated from rifampin-treated cultures."
Comments P.334 right column 2nd paragraph: "[Investigators] found that BCCP protein was stable for >60 min (Fig. 3E and F), whereas the accBC mRNA turned over with the short half-life characteristic of E. coli messages (t1/2, ca. 2 min, data not shown). These results suggested that the accBC operon was rapidly transcribed only in actively growing cells and the levels of accBC mRNA and the BCCP protein concentrations were adjusted to the rate of cell growth." See BNID 104151, 104386
Entered by Uri M
ID 102058