Halftime of hydrolysis event of DNA (3×10^9 phosphodiester bonds) at 25°C

Value 20 min
Organism Human Homo sapiens
Reference Wolfenden R, Snider MJ. The depth of chemical time and the power of enzymes as catalysts. Acc Chem Res. 2001 Dec34(12):938-45. p.940 table 1 Table - link PubMed ID11747411
Primary Source Wolfenden, R. Ridgway, C. Young, G. Spontaneous Hydrolysis of Ionized Phosphate Monoesters and Diesters and the Proficiencies of Phosphatases and Phosphodiesterases as Catalysts. J. Am. Chem. Soc. 1998, 120, 6814-6815.
Method Reaction rates were measured at series of elevated temperatures, following them to completion. The resulting Arrhenius plot, if linear, can then be extrapolated to 25 °C. In much of this work, reaction mixtures were sealed in quartz, heated over various intervals, and then opened for analysis by proton NMR. The vapor pressure of water does not change enough to affect reaction rates significantly, but aqueous samples tend to burst at temperatures above 250 °C (40 atm). Explosions are avoided by placing reaction tubes in water inside a steel bomb, equalizing pressure across the wall of each tube. PTFE vessels must be used for alkaline solutions which attack quartz.
Comments Value calculated by dividing halftime of spontaneous DNA hydrolysis at 25°C, 140000 years (BNID 105355) by number of nucleotides in human genome. Note-the halftimes of cleavage event at 25°C and 100°C (20 min and 0.23 sec, see calculation above) are different than those given in table link, however: 1) They match other cleavage event halftimes in table calculated in the same way, and (2) The 25°C value (20 min) is that given in article text, p. 940, left column 2nd paragraph. Table seems to be in error in these two values.
Entered by Uri M
ID 105358