| Method |
"The growth yields of Escherichia coli on glucose, lactose, galactose, maltose, maltotriose, and maltohexaose were estimated under anaerobic conditions in the absence of electron acceptors... Measurements of ß-galactosidase activity: The method of
Miller (17) was used except that MMA was substituted for Z
buffer. Activities are quoted in units of micromoles of
o-nitrophenol produced per minute per milligram (dry
weight) of cells. Qualitative analysis of fermentation products: Fermentation
products in culture supernatants were analyzed by the
method described in The Anaerobe Laboratory Manual (12)
with a Perkin-Elmer Fil gas chromatograph with output
from a flame ionization detector." |