Summary of nuclear and cell size distributions in asynchronous cultures

Range Table - link
Organism Budding yeast Saccharomyces cerevisiae
Reference Jorgensen P, Edgington NP, Schneider BL, Rupes I, Tyers M, Futcher B. The size of the nucleus increases as yeast cells grow. Mol Biol Cell. 2007 Sep18(9):3523-32. p.3527 table 2PubMed ID17596521
Method "All cells in a field were quantified unless they were undergoing anaphase or cytokinesis, that is, if they possessed an elongated nucleus or two separated nuclei. For all experiments, multiple cell fields were imaged. The resulting data were analyzed in Microsoft Excel (Redmond, CA) and in Matlab (MathWorks, Natick, MA). For volume estimates, it was assumed that nuclei and G1 cells were spherical and that measured areas were cross-sections through the centers of these spheres."
Comments "The N/C [nucleus/cell] ratio determined from population averages was quite constant, such that nuclear volume was 6–8% of cell volume, even though these alterations in CLN3 activity generated twofold differences in average cell volume (Table 2)." Table gives values of nuclei and cell volumes for wild type and several mutant strains. See Nuclear volume of 2.9µm^3 BNID 100447
Entered by Uri M
ID 104709