Concentration of Lac repressor

Value 50 nM
Organism Bacteria Escherichia coli
Reference Bintu L, Buchler NE, Garcia HG, Gerland U, Hwa T, Kondev J, Kuhlman T, Phillips R. Transcriptional regulation by the numbers: applications. Curr Opin Genet Dev. 2005 Apr15(2):125-35PubMed ID15797195
Primary Source Oehler S, Amouyal M, Kolkhof P, von Wilcken-Bergmann B, Müller-Hill B. Quality and position of the three lac operators of E. coli define efficiency of repression. EMBO J. 1994 Jul 15 13(14):3348-55PubMed ID8045263
Method Researchers investigated cooperative repression of the Escherichia coli lac operon by systematic conversion of its three natural operators (O1, O2 and O3) on the chromosome.
Comments This concentration corresponds to ~50 repressors per cell (BNID 102 068). 20 monomers per lacI gene given in Elf et al, 2007, PMID 17525339. For concentration of 10 nM see BNID 102083.
Entered by Uri M
ID 103602