Redox potential of cytosolic glutathione

Value -289 mV
Organism Budding yeast Saccharomyces cerevisiae
Reference Østergaard H, Tachibana C, Winther JR. Monitoring disulfide bond formation in the eukaryotic cytosol. J Cell Biol. 2004 Aug 2 166(3):337-45. abstract & p.339 table IPubMed ID15277542
Method "[Investigators] apply a previously designed GFP-based sensor for disulfide bond formation (Ostergaard et al., 2001) to study the cytosolic thiol-disulfide redox status." Using a genetically encoded glutathione-specific redox probe the concentrations of GSH and GSSG in the yeast cytosol have been estimated
Comments "For the first time a genetically encoded probe is used to determine the redox potential specifically of cytosolic glutathione. We find it to be -289mV, indicating that the glutathione redox status is highly reducing and corresponds to a cytosolic GSSG level in the low micromolar range." Table 1-Cytosolic redox potentials (E'cytosol) were calculated from the pulse-chase end point redox distributions given in Figs. 2 and 3 and an E°' of –265 mV for rxYFP
Entered by Uri M
ID 103543