Effective diffusion coefficient of Wingless (Wg) in the wing imaginal disk at 25˚C

Value 0.05 µm^2/sec Range: ±0.04 µm^2/sec
Organism Fruit fly Drosophila melanogaster
Reference Kicheva A, Pantazis P, Bollenbach T, Kalaidzidis Y, Bittig T, Jülicher F, González-Gaitán M. Kinetics of morphogen gradient formation. Science. 2007 Jan 26 315(5811):521-5. Supporting Online Material p.34/35 Supplementary table S1PubMed ID17255514
Method FRAP-Fluorescence recovery after photobleaching
Comments Supporting Online Material p.13 bottom paragraph "Modified FRAP assays: In order to test the validity of the diffusion and degradation description for the FRAP recoveries, [investigators] performed FRAP experiments in different geometries, such as different ROI [rectangular region of interest] shapes and measurements at different distances from the source. [They] find that the calculated recovery curves for all situations considered are still in good agreement with the experimental data and they yield similar values for D [µm^2/s], k [[s^-1]×10^-4], ? [molec./s×cell] and ? (Figure S4 and Table S1)."
Entered by Tobias Bollenbach
ID 101072