Levels of polyP in mitochondria

Range 0.022-0.44 Table - link µmol P/mg protein
Organism Budding yeast Saccharomyces cerevisiae
Reference Kulakovskaya TV, Lichko LP, Vagabov VM, Kulaev IS. Inorganic polyphosphates in mitochondria. Biochemistry (Mosc). 2010 Jul75(7):825-31. link p.826 table 1 PubMed ID20673205
Primary Source [25] Pestov NA, Kulakovskaya TV, Kulaev IS. Inorganic polyphosphate in mitochondria of Saccharomyces cerevisiae at phosphate limitation and phosphate excess. FEMS Yeast Res. 2004 Mar4(6):643-8. [26] Pestov, N. A. (2004) Polyphosphates and Exopolyphosphatases of Mitochondria of Yeast Saccharomyces cerevisiae: PhD Thesis [in Russian], Pushchino. [27] Andreeva NA, Kulakovskaya TV, Kulakovskaya EV, Kulaev IS. Polyphosphates and exopolyphosphatases in cytosol and mitochondria of Saccharomyces cerevisiae during growth on glucose or ethanol under phosphate surplus. Biochemistry (Mosc). 2008 Jan73(1):65-9.PubMed ID15040953, 18294131
Method P.826 right column 2nd paragraph: "Later, the polyP from isolated mitochondria of S. cerevisiae was studied by chemical extraction with 0.5 N HClO4 [perchloric acid] and electrophoresis in polyacrylamide gel [primary sources]."
Comments P.826 right column 2nd paragraph: "[Promitochondria] contained polyP at a level strongly dependent on Pi concentration in the culture medium (Table 1)." P.826 right column 3rd paragraph: "Under phosphate overplus, this level was nearly twofold higher than in the complete medium without preliminary Pi starvation (Table 1). The chain length of acid_soluble polyP of mitochondria was estimated by electrophoresis. Under Pi limitation, their polyP level was too low to be detected by this method. The chain length was <15 phosphate residues. This is close to the value determined by 31P_NMR [ref 24]. Under phosphate overplus, the mitochondrial polyP chains became longer. At the same time, polyP accumulation in mitochondria was not observed during cultivation on ethanol under aeration, even under polyP overplus in the cells [primary source 27]. Their content was extremely low (Table 1)."
Entered by Uri M
ID 105836