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||This figure was estimated by comparing plate counts
with direct microscopic counts of microorganisms in environmental
samples-it has been called the “great plate-count
anomaly” [Primary source]
||[primary source, p. 325] 'Indeed, as a general rule researchers have found
that the maximum recovery of heterotrophic bacteria is 1% of the total direct count using plating procedures or other viable enumeration methods from a variety of oligotrophic to mesotrophic aquatic habitats, whereas higher recoveries (approaching 80-90%) can be achieved from eutrophic habitats [refs 100 and 107 in Primary source]'. On p. 327 of primary source fig. 1 and 2 show a 2 order of magnitude difference between the number of bacteria (at a lake) arrived at by direct microscopic count and the number of bacteria arrived at by viable plate count.