Molecular mass of RNA polymerase core enzyme (binary subunit)

Value 4.2e+5 Da Range: ±30,000 Da
Organism Bacteria Escherichia coli
Reference Ruet A, Sentenac A, Fromageot P. On the liberation of sigma and the molecular weight of E. coli RNA polymerase. FEBS Lett. 1970 Dec11(3):169-171. doi:10.1016/0014-5793(70)80520-8 p.170 left column bottom paragraphPubMed ID11945478
Method "To estimate the molecular weights of RNA polymerase, the core enzyme and the template bound enzyme excised by DNase, their electrophoretic mobility was measured at different gel concentration as described by Hedrick and Smith [1968, PMID 5671059]."
Comments “From the calibration curve given in fig.2, the molecular weight of RNA polymerase is estimated as 480,000±30,000 daltons, whereas the core enzyme is oligomeric and runs as a protein heavier than 800,000 daltons. The excised binary complex is estimated as 420,000±30,000 daltons and the s subunit 80,000±5,000 daltons. The DNase excited initiation complex corresponding to RNA polymerase incubated with T4 DNA and ATP, UTP, CTP, behaves also as a material of 420,000 daltons, interestingly enough just as the DNase treated complex between core enzyme and T4 DNA.”
Entered by Uri M
ID 104927