Transcription error rate over all conditions (corrected value)

Value 8.23E-05 per nucleotide Range: ±0.55×10^-5 per nucleotide
Organism Bacteria Escherichia coli
Reference Traverse CC, Ochman H. Conserved rates and patterns of transcription errors across bacterial growth states and lifestyles. Proc Natl Acad Sci U S A. 2016 Mar 22 113(12):3311-6. doi: 10.1073/pnas.1525329113 Correction PMID 27402746 link 2nd paragraph PubMed ID26884158
Comments P.3311 right column 3rd paragraph to p.3312 left column top paragraph (but see updated value in "correction" at bottom): "Resource Limitation and Growth Phase Do Not Alter Transcription Error Rates: [Investigators] tested the effects of different growth conditions—all of which have been associated with altered mutation rates and/or translation error rates—on rates of transcription errors. Using a deep-sequencing approach to identify errors, [they] measured the transcription error rate in E. coli when grown under four growth conditions [tryptic soy broth (TSB) complex media or M9 glucose minimal media, each sampled at midlog and at stationary phase]. Note that these errors include both base substitutions during the process of transcription and any damage to the mRNA after transcription. Each of the four conditions were assayed in duplicate, and in total, [they] detected 2,621 transcription errors, with the number of errors per sample ranging from 156 to 681. In neither of the nutrient sources was there significant differences in transcription error rates for cells harvested at midlog phase or at 8 h after entering stationary phase (Fig. 1, paired Wilcoxon test, P > 0.30). Similar to what [they] observed for E. coli assayed at different growth phases, transcription error rates do not differ significantly in nutrient-rich (TSB) and nutrient-poor (M9) growth media (Fig. 1, paired Wilcoxon test, P = 0.3429). Furthermore, there are no significant differences in overall transcription error rates between any pair of individual conditions tested [Fig. 1, two-tailed t tests, t(2) < 2.3, P > 0.14], and the average transcription error rate over all conditions is 4.63 ± 0.34 (SEM) × 10^−5 for E. coli mRNA." See Correction PMID 27402746 link
Entered by Uri M
ID 113572