| Range |
in isolated rabbit reticulocytes 50%: in cultured mice ascites tumour cells 36% %
|
| Organism |
Mammals |
| Reference |
Brown GC. Total cell protein concentration as an evolutionary constraint on the metabolic control distribution in cells. J Theor Biol. 1991 Nov 21 153(2):195-203. p.196 bottom paragraphPubMed ID1787736
|
| Primary Source |
Siems W, Dubiel W, Dumdey R, Müller M, Rapoport SM. Accounting for the ATP-consuming processes in rabbit reticulocytes. Eur J Biochem. 1984 Feb 15 139(1):101-7 AND Müller M, Siems W, Buttgereit F, Dumdey R, Rapoport SM. Quantification of ATP-producing and consuming processes of Ehrlich ascites tumour cells. Eur J Biochem. 1986 Dec 15 161(3):701-5.PubMed ID6421575, 2947801
|
| Method |
Primary source Müller et al. abstract: "ATP production of Ehrlich ascites tumour cells was estimated on the basis of their coupled respiration and lactate formation. ATP-consuming processes were assessed from the effects of selective inhibitors of RNA synthesis, protein synthesis and proteolysis, Na+/K+-ATPase on respiration. The extent of protein synthesis and proteolysis were also determined directly." |
| Comments |
P.196 bottom paragraph: "ATP hydrolysis is required for protein synthesis and degradation. In the absence of growth cellular protein is continuously turned over, requiring a significant ATP consumption simply to maintain protein levels. In vitro this ATP usage has been
estimated to be 50% of the total ATP turnover in isolated reticulocytes (primary source Siems et al., 1984) and 36% in cultured ascites tumour cells (primary source Muller et al., 1986)." Primary source Siems et al. studied rabbits, primary source Müller et al. studied mice. |
| Entered by |
Uri M |
| ID |
113243 |