Percent of HLA-1 ligands that are proteasome-generated spliced peptides

Range 20-25 %
Organism Human Homo sapiens
Reference Liepe, J., Marino, F., Sidney, J., Jeko, A., Bunting, D. E., Sette, A., Kloetzel, P. M., Stumpf, M. P. H., Heck, A. J. R., and Mishto, M. (2016). A large fraction of HLA class I ligands are proteasome-generated spliced peptides. Science (80-. ). 354, 605–610.PubMed IDPMC4822137
Method Fig 2B, page 356. "Distribution of MS (mass spectrometry) ion peak area of spliced and nonspliced peptides eluted from the HLA-I molecules of GR-LCL (GR lymphoblastoid cell line) and C1R cell lines or human fibroblasts. Dashed lines indicate the median of the distribution for spliced peptides (blue) and nonspliced peptides (red). The MS ion peak area distribution of the nonspliced peptides is significantly larger than the distribution of the spliced peptides (Kolmogorov-Smirnov test, P<10^−16). The relative proportion of spliced peptides estimated from the integral of the MS ion peak areas of spliced peptides relative to the integral of the peak area of all peptides is reported." HLA=human leukocyte antigen
Comments This value represents the fraction of total peptide molecules observed on cell and primary cell MHC (major histocompatibility complex) complexes (HLA-1) that were identified as being spliced using the mass-spec techniques described.
Entered by Avi Flamholz
ID 113010