Translational diffusion coefficient of actin filament of 30 subunits

Value 5.2 µm^2/sec
Organism Fission yeast Schizosaccharomyces pombe
Reference Chen Q, Pollard TD. Actin filament severing by cofilin dismantles actin patches and produces mother filaments for new patches. Curr Biol. 2013 Jul 8 23(13):1154-62. doi: 10.1016/j.cub.2013.05.005. p.8 left column 2nd paragraphPubMed ID23727096
Method "[Researchers] used quantitative fluorescence microscopy to track mGFP-tagged proteins, including early endocytic adaptor proteins, activators of Arp2/3 complex, and actin filaments." See - link
Comments "Simulations of actin patch dynamics [7] estimated that severing releases short actin filaments at a prodigious rate of ~30 per second from each >100 actin patches. The cytoplasmic concentration of actin in such fragments would rapidly reach the micromolar range, even if further severing and polymerization turn over the fragments in 10 s. A filament of 30 subunits has a translational diffusion coefficient of 5.2µm^2/sec (see Experimental Procedures - link) and shorter fragments move even faster, so they diffuse rapidly throughout the cytoplasm. These short, rapidly diffusing filaments [7, 44] are difficult to distinguish from cytoplasmic actin monomers in cells with a small fraction of the total actin tagged with mGFP. Severing of filaments in actin cables nucleated by formin For3p may provide a second source of actin filament fragments [45]."
Entered by Uri M
ID 111112