Resistance & capacitance of spherical thin lipid membrane preparations of red cells in vitro

Range resistance 0.1 to 1.5 x 10^8Ω×cm^2 capacitance 0.3 to 0.9µF/cm^2.
Organism Human Homo sapiens
Reference Jung, C. Y. 1971. Permeability of bimolecular membranes made from lipid extracts of human red cell ghosts to sugar. J. Membrane Biol. 5: 200-214. p.204 bottom paragraph & p.205 top paragraphPubMed ID24173101
Method "in vitro spherical thin lipid membranes were made from the total lipid extracts, as well as from each of the major lipid constituents - phosphatidyl choline, phosphatidyl ethanolamine, sphingomyelin, and cholesterol-of human red cell membranes. All of these membrane preparations underwent a thinning process lasting about 10 to 20 min as revealed by interference colors typical of white light reflected from a thin film, followed by a rapid blackening in spots at first and then ultimately of the entire free surface. The preparations withstood gentle stirring and were stable for 1 to 2 hr."
Comments "The DC resistance of these preparations was initially relatively low, then increased gradually with time reaching a stable value of 0.1 to 1.5x10^8?xcm^2 at about 20 min. The capacitance of the membranes at the completion of the blackening was measured to be 0.3 to 0.9µF/cm^2. Both the conductance and the capacitance were increased linearly as the membrane area was increased. All of these findings agree closely with those reported by others for other bimolecular lipid membranes (Tien & Diana, 1968)."
Entered by Uri M
ID 110802