Time for RNAP assembly intermediates to appear in the nucleoid

Range β subunit ~5: β' subunit 2.5 min
Organism Bacteria Escherichia coli
Reference Klumpp S, Hwa T. Growth-rate-dependent partitioning of RNA polymerases in bacteria. Proc Natl Acad Sci U S A. 2008 Dec 23 105(51):20245-50. doi: 10.1073/pnas.0804953105. p.20246 right column top paragraphPubMed ID19073937
Primary Source Saitoh T, Ishihama A (1977) Biosynthesis of RNA polymerase in Escherichia Coli. VI. Distribution of RNA-polymerase subunits between nucleoid and cytoplasm. J Mol Biol 115: 403– 416.PubMed ID338912
Comments "Intermediates of RNAP assembly (immature RNAPs) have to be taken into account (13), because the RNAP content of cells is determined by measuring the fraction of total protein mass that is in ß and ß' subunits of RNAP (4). Some of these subunits are not or are only partially assembled into functional RNAPs in the cell (primary source). These assembly intermediates are located in the cytoplasm and radioactively labeled ß and ß' subunits appear in the nucleoid after ˜5 and 2.5 min, respectively (primary source). The larger of these times is likely to correspond to the time needed to fully assemble an RNAP plus the transition to the nucleoid, thereby providing an upper bound for the maturation time."
Entered by Uri M
ID 110062