Time required for the degradation of ubiquitinated Ub5-DHFR by mammalian 26S proteasomes

Value 13 Sec Range: Table - link Sec
Organism Mammals
Reference Peth A, Nathan JA, Goldberg AL. The ATP costs and time required to degrade ubiquitinated proteins by the 26 S proteasome. J Biol Chem. 2013 Oct 4 288(40):29215-22. doi: 10.1074/jbc.M113.482570 p.10 Table 1PubMed ID23965995
Method "Initial experiments used 26S proteasomes gently isolated from yeast (16) to determine whether they hydrolyze ATP by a stochastic or a highly cooperative manner, such as the cyclical, ordered mechanism proposed recently in which nucleotides bind and exchange in pairs (13)."
Comments See note beneath table. With folic acid added degradation time is 23 sec.
Entered by Uri M
ID 109850