Summary of FRAP experiments and model fit parameters

Range Table - link
Organism Bacteria Escherichia coli
Reference Kumar M, Mommer MS, Sourjik V. Mobility of cytoplasmic, membrane, and DNA-binding proteins in Escherichia coli. Biophys J. 2010 Feb 17 98(4):552-9. p.553 table 1PubMed ID20159151
Method "[Researchers] used fluorescence recovery after photobleaching (FRAP) along with a detailed numerical model that simulates FRAP experiments, to systematically investigate mobility of cytoplasmic, inner membrane, and DNA-binding proteins in Escherichia coli." "To characterize protein mobility in the E. coli cytoplasm and in the cytoplasmic membrane, FRAP experiments were performed for a series of 27–250 kDa cytoplasmic protein fusions to yellow and cyan fluorescent proteins (YFP and CFP, respectively), and of membrane protein fusions with 4–16 transmembrane helices ( Table 1 and Table S1)."
Comments See notes beneath table
Entered by Uri M
ID 107985