pH in mitochondrial matrix in Human endothelial cell ECV304

Value 7.78 pH units Range: ±0.17 pH units
Organism Human Homo sapiens
Reference Porcelli AM, Ghelli A, Zanna C, Pinton P, Rizzuto R, Rugolo M. pH difference across the outer mitochondrial membrane measured with a green fluorescent protein mutant. Biochem Biophys Res Commun. 2005 Jan 28 326(4):799-804. doi:10.1016/j.bbrc.2004.11.105 p.801 right column 2nd paragraphPubMed ID15607740
Method "[Researchers] generated a EYFP targeted to mitochondrial intermembrane space IMS (MIMS-EYFP) to determine for the first time the pH within this mitochondrial compartment in intact cells."
Comments "When the average values of fluorescence in the selected areas were plotted against pH, the calibration line shown in Fig. 2B was obtained. A similar calibration was carried out also in cells transfected with cyt-EYFP and with mt-EYFP (data not shown). With this calibration, the pH values obtained were 6.88 ± 0.09 (n = 32) in the IMS, 7.78 ± 0.17 (n = 9) in the matrix, and 7.59 ± 0.01 (n = 21) in the cytosol, as illustrated in Fig. 3."
Entered by Uri M
ID 107272