||Suzuki T et al., Development of a recombinant adenovirus vector production system free of replication-competent adenovirus by utilizing a packaging size limit of the viral genome. Virus Res. 2011 Jun158(1-2):154-60 abstract and p.156 left column 2nd paragraphPubMed ID21470569
||Bett AJ, Prevec L, Graham FL. Packaging capacity and stability of human adenovirus type 5 vectors. J Virol. 1993 Oct67(10):5911-21.PubMed ID8371349
||"In a conventional adenovirus (Ad) vector production method using 293 cells, homologous recombination
between Ad vector DNA and 293 cell-derived AdE1 DNA occurs with low efficiency, resulting in the generation of replication-competent adenovirus (RCA). RCA can induce the spread of replication-incompetent
Ad vectors, leading to unexpected tissue damage. In order to overcome this problem, [researchers] developed an Ad vector production system free of RCA generation by utilizing the Ad packaging size limit of the viral
||"It is well known that up to approximately 105% (37.7kb) of the wild-type genome (35.9kb) can be packaged in the Ad virion...(Bett et al., 1993)."