| Range |
Table - link
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Talia, S. D., J. M. Skotheim, J. M. Bean, E. D. Siggia, and F. R. Cross, 2007. The effects of molecular noise and size control on variability in the budding yeast cell cycle. Nature 448:947–951. Supplementary information p. 20 table S12PubMed ID17713537
|
| Method |
P.947 left column bottom paragraph: "[Researchers] measured times from cytokinesis to budding (G1) and from budding to cytokinesis in haploids, diploids or tetraploids (mothers and daughters), using time-lapse fluorescence microscopy of strains expressing Myo1 tagged with green fluorescent protein (Myo1–GFP)." P.950 left column paragraph above bottom: "Strain and plasmid constructions: Standard methods were used throughout. All strains are W303-congenic. All integrated constructs were characterized by Southern blot analysis. Cells were prepared for time-lapse microscopy as described (ref 24, Bean et al. 2006 PMID 16387649). [Researchers] observed growth of microcolonies with fluorescence time-lapse microscopy at 30°C using a Leica DMIRE2 inverted microscope with a Ludl motorized XY stage. Images were acquired every 3min for cells grown in glucose and every 6min for cells grown in glycerol/ethanol with a Hamamatsu Orca-ER camera. [They] used custom Visual Basic software integrated with ImagePro Plus to automate image acquisition and microscope control." |
| Comments |
Media and temperature dependent. Although unspecified, the growth media appears to be glucose according to following sentence from p.16 in supplementary information: "Glycerol/ethanol supports a much slower growth rate than glucose (170 min compared to 100 min doubling time)..." See BNID 101310, 104360. For 100 min in rich medium see BNID 100270 |
| Entered by |
Uri M |
| ID |
105487 |