Table - link g/kg dry matter
||Shaw AJ et al., Metabolic engineering of microbial competitive advantage for industrial fermentation processes. Science. 2016 Aug 5 353(6299):583-6. doi: 10.1126/science.aaf6159. Supplementary Materials p.25 table S3PubMed ID27493184
||See refs beneath table
||Abstract: "[Investigators] engineered Escherichia coli to assimilate melamine, a xenobiotic compound containing nitrogen. After adaptive laboratory evolution to improve pathway efficiency, the engineered strain rapidly outcompeted a control strain when melamine was supplied as the nitrogen source. [They] additionally engineered the yeasts Saccharomyces cerevisiae and Yarrowia lipolytica to assimilate nitrogen from cyanamide and phosphorus from potassium phosphite, and they outcompeted contaminating strains in several low-cost feedstocks."
||P.585 left column bottom paragraph: "[Investigators] evaluated sugarcane juice and wheat straw lignocellulosic hydrolysate, two broadly available industrial feedstocks, for ROBUST fermentation with S. cerevisiae strain NS586. Sugarcane juice is composed primarily of simple sugars, but also contains some free amino acid–bound nitrogen and 0.1 to 0.6 g of phosphorus per kilogram of dry matter, or 1.7 to 12.5% of the phosphorus necessary for complete aerobic conversion of the sugars to yeast biomass (table S3)." P.585 middle column bottom paragraph: "Wheat straw lignocellulosic hydrolysate is under evaluation for the production of bioethanol and biochemicals, and it requires nitrogen supplementation for complete growth of S. cerevisiae (fig. S9). [Investigators] co-inoculated S. cerevisiae NS586 and K. marxianus CBS 6556 at a 10:1 initial ratio with 5 mM urea or cyanamide in 2% w/v glucan-equivalent wheat straw hydrolysate. Although the cyanamide condition has a 4- to 5-hour lag relative to the urea condition, it enables S. cerevisiae NS586 both to grow unhindered by K. marxianus (Fig. 3B) and to achieve a higher cellulosic ethanol titer (fig. S10). Other industrial feedstocks such as sugarcane bagasse, sugar beet molasses, and corn stover have comparable limiting phosphorus and nitrogen contents (table S3), making them feasible for further ROBUST processing." See notes above and beneath table