Parameters in study of mitochondrial content in skeletal muscle

Range Table - link
Organism Human Homo sapiens
Reference Larsen S et al., Biomarkers of mitochondrial content in skeletal muscle of healthy young human subjects. J Physiol. 2012 Jul 15 590(14):3349-60. doi: 10.1113/jphysiol.2012.230185. p.3354 table 2PubMed ID22586215
Method Abstract: "Sixteen young healthy male subjects were recruited for this study. Subjects completed a graded exercise test to determine maximal oxygen uptake (VO2peak) and muscle biopsies were obtained from the vastus lateralis. Mitochondrial content was determined using transmission electron microscopy imaging and OXPHOS [muscle oxidative capacity] was determined as the maximal coupled respiration in permeabilized fibres."
Comments P.3354 left column bottom paragraph: "Correlation between the biomarkers and the mitochondrial fractional area: Several biomarkers showed a strong correlation and high concordance with mitochondrial fractional area. The correlation coefficients, P values and Lin concordance coefficients can be seen in Table 2 where the biomarkers are listed according to their Rc rank to the mitochondrial fractional area. Cardiolipin content had the strongest correlation and highest Rc value of 0.85 defined as an ‘almost perfect concordance’ (Lin, 1989 Fig. 3A)." P.3355 left column 2nd paragraph:"The cristae surface area was significantly correlated with the same biomarkers as the mitochondrial content (Table 2). The biomarker that showed the strongest concordance with cristae surface area was complex I activity (Rc= 0.71) and complex II protein content (Rc= 0.71), followed by complex V protein content (Rc= 0.70), cardiolipin content (Rc= 0.67), CS [citrate synthase] activity (Rc= 0.67) and complex IV activity (Rc= 0.64). As can be seen in Table 2, the concordance was generally lower between the biomarkers and the cristae surface area when compared with mitochondrial surface area." See notes beneath table
Entered by Uri M
ID 112455