Proportion of synapses from different synaptic subtypes

Range glutamatergic ~84%: GABAergic ~16% Table - link %
Organism Mouse Mus musculus
Reference Micheva KD, Busse B, Weiler NC, O'Rourke N, Smith SJ. Single-synapse analysis of a diverse synapse population: proteomic imaging methods and markers. Neuron. 2010 Nov 18 68(4):639-53. doi: 10.1016/j.neuron.2010.09.024. p.649 table 2PubMed ID21092855
Method Abstract:"[Investigators] describe the use of array tomography, a new high-resolution proteomic imaging method, to determine the composition of glutamate and GABA synapses in somatosensory cortex of Line-H-YFP Thy-1 transgenic mice."
Comments p.646 right column bottom paragraph:"Thus, in addition to the presence of a ubiquitous synaptic marker (e.g., synapsin or bassoon) and the cytoplasmic GAD, [investigators] required the presence of the vesicular marker VGAT, which has been shown to specifically localize to presynaptic boutons (Chaudhry et al., 1998). The results obtained from these two experiments were very similar (Table 2). Approximately 84% synapses were glutamatergic and 16% GABAergic. There were almost twice as many VGluT2 containing synapses (VGluT1 + 2 subtype and VGluT2 only subtype) in layer 4 compared to layer 5 (21.4% versus 12.9%)."
Entered by Uri M
ID 112025