Nucleic acid and protein content of S. cerevisiae, and cell volume

Range Table - link
Organism Budding yeast Saccharomyces cerevisiae
Reference Weiss RL, Kukora JR, Adams J. The relationship between enzyme activity, cell geometry, and fitness in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1975 Mar72(3):794-8. p.796 table 1PubMed ID1093169
Method P.795 right column 2nd paragraph: "Nucleic Acid and Protein Assays: DNA was extracted from a determined number of cells (1 to 5×10^9) with trichloroacetic acid as previously described (ref 4). DNA was determined in the trichloroacetic acid extract by a modified diphenylamine procedure (refs 12, 13). Standard curves were prepared with salmon sperm DNA (Mann). RNA was extracted and assayed as described by Ogur and Rosen (ref 14) with yeast RNA (Sigma) as a standard. Total protein was determined (ref 15) after extraction of a determined number of cells with 1 N NaOH for 24 hr, with bovine serum albumin as a standard."
Comments P.795 right column bottom paragraph: "Macromolecular Composition: The macromolecular composition of haploid and diploid cells was examined during both unlimited and carbon-limited growth. Diploid cells under unlimited (all components of the medium present in excess) growth conditions have 1.57 the volume of haploid cells. However, when diploid cells are grown under conditions of carbon-limitation, they possess exactly the same volume as haploid cells (Table 1). Thus, by varying the conditions of the environment [investigators] can vary cell size independent of the level of ploidy. The DNA contents of such cells are shown in Table 1."
Entered by Uri M
ID 109469