| Value |
550
nM
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Bintu L, Buchler NE, Garcia HG, Gerland U, Hwa T, Kondev J, Phillips R. Transcriptional regulation by the numbers: models. Curr Opin Genet Dev. 2005 Apr15(2):116-24. Fig. 1PubMed ID15797194
|
| Primary Source |
[38] Liu M, Gupte G, Roy S, Bandwar RP, Patel SS, Garges S. Kinetics of transcription initiation at lacP1. Multiple roles of cyclic AMP receptor protein. J Biol Chem. 2003 Oct 10 278(41):39755-61PubMed ID12881519
|
| Method |
Primary source abstract: "[Researchers] examined the kinetics of open complex formation at the lacP1 promoter using tryptophan fluorescence of RNA polymerase and DNA fragments with 2-aminopurine substituted at specific positions." |
| Comments |
P.118 caption to fig.1c: "In particular, making the simplest assumption that the genomic background for RNAP is given only by the non-specific binding of RNAP with DNA, [investigators] take K[NS]pd=10 000 nM [ref 37], for the lac promoter K[S]pd=550 nM [primary source] and for the T7 promoter, K[S]pd=3nM [BNID 103592]. For the lac promoter, this results in Δɛpd=-2.9kBT and for the T7 promoter, Δɛpd=-8.1kBT." |
| Entered by |
Uri M |
| ID |
103590 |