Constant for binding of RNAP to lac promoter

Value 550 nM
Organism Bacteria Escherichia coli
Reference Bintu L, Buchler NE, Garcia HG, Gerland U, Hwa T, Kondev J, Phillips R. Transcriptional regulation by the numbers: models. Curr Opin Genet Dev. 2005 Apr15(2):116-24. Fig. 1PubMed ID15797194
Primary Source [38] Liu M, Gupte G, Roy S, Bandwar RP, Patel SS, Garges S. Kinetics of transcription initiation at lacP1. Multiple roles of cyclic AMP receptor protein. J Biol Chem. 2003 Oct 10 278(41):39755-61PubMed ID12881519
Method Primary source abstract: "[Researchers] examined the kinetics of open complex formation at the lacP1 promoter using tryptophan fluorescence of RNA polymerase and DNA fragments with 2-aminopurine substituted at specific positions."
Comments P.118 caption to fig.1c: "In particular, making the simplest assumption that the genomic background for RNAP is given only by the non-specific binding of RNAP with DNA, [investigators] take K[NS]pd=10 000 nM [ref 37], for the lac promoter K[S]pd=550 nM [primary source] and for the T7 promoter, K[S]pd=3nM [BNID 103592]. For the lac promoter, this results in Δɛpd=-2.9kBT and for the T7 promoter, Δɛpd=-8.1kBT."
Entered by Uri M
ID 103590