Gibbs free energy for redox transfer across the Inner Chloroplast Envelope Membrane

Value -13 kJ/mol
Organism Spinach Spinacia oleracea
Reference Heineke D, Riens B, Grosse H, Hoferichter P, Peter U, Flügge UI, Heldt HW. Redox Transfer across the Inner Chloroplast Envelope Membrane. Plant Physiol. 1991 Apr95(4):p. 1136PubMed ID16668101
Method Hydroponic growth. Spinach plants were grown in a climatized chamber with a 9 h light/15 h dark cycle and leaves were stopped in liquid N2 after 8.5 h light or 2 h dark. The frozen tissue was ground and lyophilized. The dry material was homogenized by sonication in a mixture of carbon tetrachloride and heptane, and fractionated by density gradient centrifugation.
Comments between NADPH/NADP in stroma and NADH/NAD in cytosol. Assuming a stromal pH of 8.0 and cytosolic pH of either 7.2 or 7.6
Entered by Uri M
ID 103335