Translation burst size of a fusion protein

Value 4 molecules
Organism Bacteria Escherichia coli
Reference X. Sunney Xie, Paul J. Choi, Gene-Wei Li, Nam Ki Lee, and Giuseppe Lia, 'Single-Molecule Approach to Molecular Biology in Living Bacterial Cells', Annu. Rev. Biophys. 2008. 37:p.433 caption of figure 7bPubMed ID18573089
Primary Source Yu J, Xiao J, Ren X, Lao K, Xie XS. 2006. Probing gene expression in cells, one molecule at a time. Science 311:1600–3PubMed ID16543458
Method "observation of real-time production of single protein molecules in individual cells. A fusion protein of a fast-maturing yellow fluorescent protein (YFP) and a membrane-targeting peptide was expressed under a repressed condition"
Comments "Gene expression occurs in bursts, with each burst originating from a stochastically transcribed single messenger RNA molecule. Protein copy numbers in the bursts follow a geometric distribution"
Entered by Uri M
ID 102044