Release of cell walls from Staphylococcus aureus by disruption of the cells in a Braun shaker with glass beads

Range Table - link %
Organism Bacteria Staphylococcus aureus
Reference Huff E, Oxley H, Silverman CS. Density-gradient patterns of Staphylococcus aureus cells and cell walls during growth and mechanical disruption. J Bacteriol. 1964 Oct88 :1155-62. p.1159 table 1PubMed ID14219032
Method Abstract: "Procedures capable of rapid disruption of Staphylococcus aureus cells with optimal release of intact cell walls were investigated. This search was implemented by observation of the flotation patterns of cells and subcellular particulate matter after centrifugation in a cesium chloride density gradient. A quantitative evaluation of the light-scatter throughout the gradient was achieved by transfer of the entire density gradient into an optical cell with wedge-shaped cross section. When this cell was photographed under indirect illumination, each band of light-scattering material appeared on the negative as a shaded curve, with an area proportional to amount of that material present. A series of photographs of known amounts of cells and cell walls was used to estimate the amounts of these materials in mixtures of the two occurring during mechanical disruption."
Comments P.1158 right column bottom paragraph: "A representative series of CsCl (cesium chloride) gradient patterns observed during disruption of staphylococcal cells in a Braun shaker is shown in Fig. 2. The amounts of cell walls and unruptured cells were determined from these patterns by measurement of the area under the light-scatter curve and comparison with the standard curves of Fig. 3. These data are presented in Table 1." P.1159 left column 5th paragraph: "A comparison of the viable counts and unruptured cells in Table 1 shows that after 1 min of shaking the unruptured cells are predominantly nonviable. This result is in contrast to the results of Cooper (1953) who, with a mechanical shaker, found a close correlation between viable count and rate of disruption of S. aureus. In the case of the Branson 20-kc sonifier, it was observed that disappearance of the unruptured cells parallels the loss of viable count." See notes beneath table
Entered by Uri M
ID 113012