Estimated translocating distance of injected creatine kinase in squid giant axons

Range Table - link µm
Organism Squid
Reference Terada S, Kinjo M, Hirokawa N. Oligomeric tubulin in large transporting complex is transported via kinesin in squid giant axons. Cell. 2000 Sep 29 103(1):141-55. p.146 table 2PubMed ID11051554
Method P.141 right column 2nd paragraph:"[Investigators] injected labeled tubulin into squid giant axons and monitored its fluorescent profile by both confocal laser scanning microscopy (CLSM) (Figure 1A) and fluorescence correlation spectroscopy (FCS) (Figure 7A)."
Comments P.143 right column bottom paragraph:"Then, in order to rule out the possibilities that the apparent difference of measured transporting velocities between the initial and later (more than 1 hr after injection) time points might reflect any different mechanism of transport, [investigators] repeated the same kind of pharmacological experiments with creatine kinase using the long time points (Table 2). [They] measured the transporting distance of creatine kinase from injection point after 1, 2, and 3 hr postinjection. During 1 and 2 hr postinjection, [they] applied nocodazole (at the concentration of 40 μg/ml), or cytochalasin D (at the concentration of 2 μM) to the bath. As shown in Table 2, the first transporting distance per hour was not different from control specimen (control, 128.7 ± 91.1 μm [n = 3] nocodazole treatment, 127.6 ± 55.5 μm [n = 3] cytochalasin D treatment, 135.5 ± 97.3 [n = 3]), but only nocodazole treatment profoundly inhibited transport during 1 and 2 hr postinjection (control, 369.9 ± 39.1 μm [n = 3] nocodazole treatment, 222.8 ± 77.4 μm [n = 3] cytochalasin D treatment, 326.3 ± 69.7 [n = 3])." Animals studied were Heterololigo bleekeri, Todarodes pacificus pacificus, or Loligo pealei.
Entered by Uri M
ID 112177