Cell count, heat output, and heat output per cell early in the logarithmic phase and at the point of maximal heat output

Range Table - link
Organism Bacteria Escherichia coli
Reference Rothbaum HP, Stone HM. Heat output of Escherichia coli. J Bacteriol. 1961 Feb81: 172-7. p.173 table 2 & table 1PubMed ID13743863
Method p.172 left column 2nd paragraph:"For heat output measurements E. coli was grown in nutrient broth on the surface of asbestos fiber in order to obtain good aeration conditions." p.172 right column 2nd paragraph:"The temperature differential was continuously recorded on a 1 mv recorder and the outflowing air passed through a thermal conductivity type carbon dioxide analyzer, which recorded its concentration." p.172 right column 3rd paragraph:"Periodically, a small sample of asbestos was removed from the flask and the number of bacteria was estimated by vigorously shaking the sample with 100 ml of sterile Ringer's solution at 37°C for 2 min, and plating out at least three known dilutions on tryptone glucose extract agar medium (Difco)." p.173 left column top paragraph:"All heat output experiments were done in duplicate and never differed by more than 20 per cent." p.173 left column 2nd paragraph:"The water activity of broths was determined by a simple form of the isopiestic method of Robinson and Sinclair (1934)."
Comments "Table 1 [please see below table 2 in link] gives the composition, densities, and water activities of the broths used. Figure 1 shows typical curves for the heat output of broth A, B, E, and G, whereas figure 2 gives their carbon dioxide production. Table 2 shows the cell counts, total heat outputs, and heat output per cell in the same experiments early in the logarithmic phase and at the point of maximal heat output."
Entered by Uri M
ID 111501